A STUDY OF THE EFFECT OF USING A WATER-ALCOHOLIC AND NANO-BASED ONION EXTRACT AGAINST SOME PATHOGENIC MICROBES THAT CAUSE FOOD SPOILAGE

Document Type : Research Paper

Authors

1 Market Research and Consumer Protection Center, University of Baghdad, IRAQ

2 Department of Biology, College of Science, Al-Mustansiriya University

3 Market Research and Consumer Protection Center, University of Baghdad, Iraq

Abstract

As a result of the strong tendency that has emerged recently to use plants and their active components in medicine and as a result of the abundance of these plants (as most of them are used in the kitchen) and their use in traditional medicine, therefore, nanoparticles of iron oxides were manufactured by the reduction reaction of iron ions due to the presence of phenolic compounds in the onion plant. Which works as a biological carrier agent, as the diagnostic techniques used have proven the correct formation of iron oxide nanoparticles, as the scanning electron microscope (SEM) technique confirmed that the size of the particles ranged between 5-50 nanometers, while the study showed the descriptions of the prepared nanostructure using X-ray diffraction. Spectroscopy: The appearance of four diffraction peaks when creating onion peels with embedded iron particles compared to the iron nanoparticles alone, which showed the presence of three diffraction peaks when diagnosed with the same technique, while FTIR infrared spectroscopy showed the presence of different and distinct peaks for the nano-onion peels compared to the iron nanoparticles. The biological effectiveness of nano-onion peels was studied on two types of harmful bacteria, one of which was E. coli positive and the other Staphylococcus aureus negative. The results showed that there was a difference between the biological effectiveness of onion nano-peels compared to iron nanoparticles and onion peels in affecting these two strains.

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