Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions

Nooraei, P.; Shareghi, B.; Salavati-Niasari, M.; Shahbazkia, H. R.; Semnani, A.

doi:10.7508/jns.2012.01.005

Comparative Studies on the Interaction of Proteinase-K with Nano-CuO and Copper Ions

Document Type : Research Paper

Authors

P. Nooraei ¹

B. Shareghi ¹

M. Salavati-Niasari ²

H. R. Shahbazkia ¹

A. Semnani ¹

¹ Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran

² Institute of Nano Science and Nano Technology, University of Kashan, Kashan, P.O.BOX.87317-51167,I. R. of Iran

10.7508/jns.2012.01.005

Abstract

The interaction of copper oxide nanoparticles and copper ions and proteinase K from Tritirachium album Limber has been investigated employing UV spectroscopy and kinetics measurements. The aim of this study was to evaluate the effect of nanoparticles of CuO for proteinase K. In this paper we compare the effect of copper oxide nanoparticles with the effect of copper ions on proteinase K stability and activity. Copper oxide nanoparticles had more inhibitory and destabilization effect than copper ions on proteinase K. We used thermodynamic parameter such as Tm and ∆G0 and kinetic studies to emphases the effect of nanoparticles CuO and copper ions on stability and activity of proteinase-K.

Keywords

Proteinase-K

Copper oxide

Nanoparticles

Thermodynamic parameter

Nanostructures