Facile Approach to Synthesize and Characterization of Silver Nanoparticles by Using Mulberry Leaves Extract in Aqueous Medium and its Application in Antimicrobial Activity

Document Type : Research Paper

Authors

1 Department of Nanotechnology, Sri Guru Granth Sahib World University, Fatehgarh Sahib -140406, Punjab, India

2 Intelligent Material Pvt. Ltd.(Nanoshel LLC), Derabassi- 140507, Punjab, India

Abstract

There is a huge demand of silver nanoparticles in the global market due to their special properties and applications in different fields such as nanomedicine , dentists , nanocatalysis, nanoelectronics, textile field, waste water treatment.The major cons of top down and Bottom up methods are the synthesis processes are highly costly, time consuming and many harmful chemicals are used. To reduce these problems Green chemistry comes to play a very important role for making of silver nanoparticles. Use of various plant extracts like leaves, fruits for synthesis of biogenic silver nanoparticles referred as Green Nanotechnology. In the present work we reported the green synthesis of silver nanoparticles using by Mulberry leaves extract without using any toxic chemicals. The Mulberry leaves extract act as reducing agent as well as a stabilizing agent in green nanotechnology process. The making of silver nanoparticles was determined by the change of shade from white to brownish by the addition of Mulberry leaves extract . UV-Vis absorption spectroscopy was used to monitor the measurable formation of silver nanoparticles showed a maximum peak at 440 nm. High resolution Transmission electron microscope confirmed the spherical nature and the highly crystallinity of silver nanoparticles on an average size 15 -25 nm .Antimicrobial activity of the biogenic Ag nanoparticles was performed by a well diffusion method. The nanoparticles exhibited enhanced anti-bacterial activity when incubated in Escherichia Coli and Bacillus Subtilis cultured plates at varied volumes. Current study thus presents a facile and innovative strategy for synthesis of silver nanoparticles.

Keywords

References

1. Huang X, El-Sayed IH, Qian W, El-Sayed MA. Cancer Cell Imaging and Photothermal Therapy in the Near-Infrared Region by Using Gold Nanorods. J Am Chem Soc . , 2006 Feb 1;128(6):2115–20.
2. Su X-Y, Liu P-D, Wu H, Gu N. Enhancement of radiosensitization by metal-based nanoparticles in cancer radiation therapy. Cancer Biol Med. 2014;11(2):86–91.
3. O’Neal DP, Hirsch LR, Halas NJ, Payne JD, West JL. Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles. Cancer Lett. 2004;209(2):171–6.
4. Gaspar VM, Marques JG, Sousa F, Louro RO, Queiroz J a, Correia IJ. Biofunctionalized nanoparticles with pH-responsive and cell penetrating blocks for gene delivery. Nanotechnology . 2013;24:1–16.
5. Ding Y, Jiang Z, Saha K, Kim CS, Kim ST, Landis RF, et al. Gold nanoparticles for nucleic acid delivery. Mol Ther. 2014;22(6):1075–83.
6. Jain PK, Huang X, El-Sayed IH, El-Sayed MA. Review of some interesting surface plasmon resonance-enhanced properties of noble metal nanoparticles and their applications to biosystems. Plasmonics. 2007;2(3):107–18.
7. Zharov VP, Mercer KE, Galitovskaya EN, Smeltzer MS. Photothermal nanotherapeutics and nanodiagnostics for selective killing of bacteria targeted with gold nanoparticles. Biophys J . 2006;90(2):619–27.
8. U J.A. Bumzpus, M. Tien, D. Wright, and S.D. Aust, Science 228 (1985) 1434.
9. Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and their antimicrobial activities. Vol. 145, Advances in Colloid and Interface Science. 2009. p. 83–96.
10. Ge L, Li Q, Wang M, Ouyang J, Li X, Xing MMQ. Nanosilver particles in medical applications: Synthesis, performance, and toxicity. Vol. 9, Int J Nanomedicine. 2014. p. 2399–407.
11. Sterling, E., Stolk, J., Hafford, L. et al. Metall and Mat Trans A (2009) 40: 1701. 
 12. Wu ZG, Munoz M, Montero O. The synthesis of nickel nanoparticles by hydrazine reduction. Adv Powder Technol. 2010;21(2):165–8.
13. Qin Y, Ji X, Jing J, Liu H, Wu H, Yang W. Size control over spherical silver nanoparticles by ascorbic acid reduction. Colloids Surfaces A Physicochem Eng Asp. 2010;372(1-3):172–6.
14. Luo C, Zhang Y, Zeng X, Zeng Y, Wang Y. The role of poly(ethylene glycol) in the formation of silver nanoparticles. J Colloid Interface Sci. 2005;288(2):444–8.
15. Makarov V V., Love AJ, Sinitsyna O V., Makarova SS, Yaminsky I V., Taliansky ME, et al. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Vol. 6, Acta Naturae. 2014. p. 35–44.
16. Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess . 2014;1(1):1–10.
17. Geraldes AN, Alves A, Leal J, Estrada-villegas GM, Lincopan N, Katti K V, et al. Green Nanotechnology from Plant Extracts : Synthesis and Characterization of Gold Nanoparticles. Advances in Nanoparticles 2016;(August):176–85.
18. Jha AK, Prasad K, Prasad K, Kulkarni AR. Plant system: Nature’s nanofactory. Colloids Surfaces B Biointerfaces. 2009;73(2):219–23.
19. Nakkala JR, Mata R, Gupta AK, Sadras SR. Biological activities of green silver nanoparticles synthesized with Acorous calamus rhizome extract. Eur J Med Chem. 2014;85:784–94.
20. Sathishkumar P, Vennila K, Jayakumar R, Yusoff ARM, Hadibarata T, Palvannan T. Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: An effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells. Bioprocess Biosyst Eng. 2016;39(4):651–9.
21. Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanoparticle Res. 2011;13(7):2981–8.
22. Ahmed S, Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. J Radiat Res Appl Sci.2016;9(1):1–7.
23. Narayanan KB, Park HH. Antifungal activity of silver nanoparticles synthesized using turnip leaf extract (Brassica rapa L.) against wood rotting pathogens. Eur J Plant Pathol. 2014;140(2):185–92.
24. Arunachalam R, Dhanasingh S, Kalimuthu B, Uthirappan M, Rose C, Mandal AB. Phytosynthesis of silver nanoparticles using Coccinia grandis leaf extract and its application in the photocatalytic degradation. Colloids Surfaces B Biointerfaces . 2012 Jun 1;94:226–30.
25. Zargar M, Shameli K, Najafi GR, Farahani F. Plant mediated green biosynthesis of silver nanoparticles using Vitex negundo L. extract. J Ind Eng Chem. 2014;20(6):4169–75.
26. Kathiravan V, Ravi S, Ashokkumar S. Synthesis of silver nanoparticles from Melia dubia leaf extract and their in vitro anticancer activity. Spectrochim Acta - Part A Mol Biomol Spectrosc. 2014;130:116–21.
27. Yang X, Yang L, Zheng H. Hypolipidemic and antioxidant effects of mulberry (Morus alba L.) fruit in hyperlipidaemia rats. Food Chem Toxicol. 2010;48(8-9):2374–9.
28. Vijayan K, Srivastava PP, Awasthi a K. Analysis of phylogenetic relationship among five mulberry (Morus) species using molecular markers. Genome . 2004;47(3):439–48.
29. Hassimotto NMA, Genovese MI, Lajolo FM. Absorption and metabolism of cyanidin-3-glucoside and cyanidin-3-rutinoside extracted from wild mulberry (Morus nigra L.) in rats. Nutr Res. 2008;28(3):198–207.
30. Aghlara H, Rostami R, Maghoul A, SalmanOgli A. Noble metal nanoparticle surface plasmon resonance in absorbing medium. Opt - Int J Light Electron Opt . 2015;126(4):417–20.
Journal of Nanostructures
Volume 7, Issue 2
April 2017
Pages 134-140

Files

Share

How to cite

Statistics