Microwave-Assisted Synthesis of kappa-Carrageenan Beads Containing Silver Nanoparticles with Dye Adsorption and Antibacterial Properties

Document Type : Research Paper


Department of Chemistry, Payame Noor University, 19395-4697, Tehran, Iran.



In this work, we used a simple and totally green method for synthesizing silver nanoparticles using kappa-carrageenan as reducing and stabilizing agent. The beads were prepared in aqueous medium by microwave heating, and then followed by cross-linking with K+ cations without using any additional toxic and expensive chemical agents. The preparation method of the carrageenan-based beads is easy, fast, simple, effective, and safe. The synthesized beads loaded with were characterized by ultraviolet-visible absorbance spectra, transmition electron microscopy and X-Ray diffraction techniques. The as-prepared beads were evaluated to remove cationic crystal violet dye from aqueous solutions. The thermodynamic parameters shown that the sorption process was feasible, spontaneous and endothermic. The kinetics and isotherm of crystal violet adsorption were found to well fit to pseudo-second-order kinetic and Langmuir isotherm model, respectively. Moreover, the antibacterial activity of the obtained beads was examined using the nutrient agar disc diffusion method.


[1] Lee S, Ha KP. Dual pH and temperature responsive hydrogels based on â-cyclodextrin derivatives for atorvastatin delivery. Polymer(Korea). 2015; 39: 300-306.
[2] Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res. 2008; 10: 507-517.
[3] Zhao X, Xi Y, Li Q, Ma X, Quan F, Geng C, Han Zh. Microwave-assisted synthesis of silver nanoparticles using sodium alginate and their antibacterial activity. Coll Surf A: Physicochem Eng Asp. 2014; 444: 180-188.
[4] Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009; 27: 76-83.
[5] Wang L, Ren J, Zhang X, Yang X, Yang W, Synthesis and characterization of pH-sensitive and self-oscillating IPN hydrogel in a pH oscillator. Polymer(Korea). 2015; 39: 359-364.
[6] Wang H, Qiao X, Chen J, Ding S. Preparation of silver nanoparticles by chemical reduction method. Coll Surf A: Physicochem Eng Asp. 2005; 256: 111-115.
[7] Xia NX, Cai YR, Jiang T, Yao JM. Green synthesis of silver nanoparticles by chemical reduction with hyaluronan. Carbohydr Polym. 2011; 86: 956-961.
[8] Fanta GF, Kenar JA, Felker FC, Byars JA, Preparation of starch-stabilized silver nanoparticles from amylose-sodium palmitate inclusion complexes. Carbohydr Polym. 2013; 92: 260-268.
[9] Sakai H, Kanda T, Shibata H, Ohkubo T, Abe M. Preparation of highly dispersed core/shell-type titania nanocapsules containing a single Ag nanoparticle. J Am Chem Soc. 2006: 128: 4944-4945.
[10] Zaheer Z. Multi-branched flower-like silver nanoparticles: preparation and characterization. Coll Surf A: Physicochem Eng Asp. 2011; 384: 427-431.
[11] Medina-Ramirez I, Bashir S, Luo Z, Liu JL, Green synthesis and characterization of polymer-stabilized silver nanoparticles. Coll Surf B: Biointerfaces. 2009; 73: 185-191.
[12] Wei DW, Sun WY, Qian WP, Ye YZ, Ma XY. The synthesis of chitosan-based silver nanoparticles and their antibacterial activity. Carbohydr Res. 2009; 344: 2375-2382.
[13] Kassaee MZ, Akhavan A, Sheikh N, Beteshobabrud R. م-Ray synthesis of starch-stabilized silver nanoparticles with antibacterial activities. Radiat Phys Chem. 2008; 77: 1074-1078.
[14] Lv X, Yang S, Jin J, Zhang L, Li G, Jiang J. Microwave absorbing characteristics of epoxy composites containing carbon black and carbon fibers (in Korean). Polymer(Korea). 2009; 33: 420-428.
[15] Yi CF, Deng ZW, Xu ZS. Development of studies on polymerization by microwave irradiation. Polym Bull. 2004; 1: 30-36.
[16] Bilecka I, Elser P, Niederberger M. Kinetic and thermodynamic aspects in the microwave-assisted synthesis of ZnO nanoparticles in benzyl alcohol. ACS Nano. 2009; 3: 467-477.
[17] Zhu JF, Zhu YJ, Ma MG, Yang LX, Gao L. Simultaneous and rapid microwave synthesis of polyacrylamide-metal sulfide (Ag2S, Cu2S, HgS) nanocomposites. J Phys Chem. C 2007; 111: 3920-3926.
[18] Hong SY, Lee JY. pH-Dependent dye adsorption and release behaviors of poly(ethylene-alt-maleic anhydride)/poly(4-vinyl pyridine) multiplayer films. Polymer(Korea). 2005; 29: 593-598.
[19] Bagheri Marandi G, Peyvand Kermani Z, Kurdtabar M. Fast and efficient removal of cationic dyes from aqueous solution by collagen-based hydrogel nanocomposites. Polym Plast Technol Eng. 2013; 52: 310-318.
[20] Zhao S, Zhou F, Li L, Cao M, Zuo D, Liu H. Removal of anionic dyes from aqueous solutions by adsorption of chitosan-based semi-IPN hydrogel composites. Composites: Part B. 2012; 43: 1570-1578.
[21] Oladipo AA, Gazi M, Saber-Samandari S. Adsorption of anthraquinone dye onto eco-friendly semi-IPN biocomposite hydrogel: equilibrium isotherms, kinetic studies and optimization. J Taiwan Inst Chem Eng. 2014; 45: 653-664.
[22] Abdel-Halim ES, Al-Deyab SS. Utilization of hydroxypropyl cellulose for green and efficient synthesis of silver nanoparticles. Carbohydr Polym. 2011; 86: 1615-1622.
[23] Slokar YM, Le Marechal AM. Methods of decoloration of textile wastew-aters. Dyes Pigm. 1998; 37: 335-356.
[24] Madaeni SS, Jamali Z, Islami N. Highly efficient and selective transportof methylene blue through a bulk liquid membrane containing Cyanex 301 ascarrier. Sep Purif Technol. 2011; 81: 116-123.
[25] Chakraborty S, De S, DasGupta S, Basu JK. Adsorption study for the removal of a basic dye: experimental and modeling. Chemosphere. 2005; 58: 1079-1086.
[26] Ghorai S, Sarkar AK, Panda AB, Pal S. Effective removal of Congo red dye from aqueous solution using modified xanthan gum/silica hybrid nanocomposite as adsorbent. Biores Technol. 2013; 144: 485-491.
[27] Sarkar AK, Pal A, Ghorai S, Mandre NR, Pal S. Efficient removal of malachite green dye using biodegradable graft copolymer derived from amylopectin and poly(acrylic acid). Carbohyd Polym. 2014; 111: 108-115.
[28] 28. Hosseinzadeh H, Khoshnood  N. Removal of cationic dyes by poly(AA-co-AMPS)/montmorillonite nanocomposite hydrogel.    Des  Water Treat. 2015; 57: 6372–6383.
[29] Hosseinzadeh H, Mohammadi S. Quince seed mucilage magnetic nanocomposites as novel bioadsorbents for efficient removal of cationic dyes from aqueous solutions. Carbohyd Polym. 2015; 134: 213-221.
[30] Hosseinzadeh H, Zoroufi Sh, Mahdavinia GR. Study on adsorption of cationic dye on novel kappa-carrageenan/poly(vinyl alcohol)/montmorillonite nanocomposite hydrogels. Polym Bull. 2015; 72: 1339-1363.
[31] Abdel-Halim ES, Al-Deyab SS. Utilization of hydroxypropyl cellulose for green and efficient synthesis of silver nanoparticles. Carbohydr Polym. 2011; 86: 1615-1622.
[32] Yang S, Wang Y, Wang Q, Zhang R, Ding B. UV irradiation induced formation of Au nanoparticles at room temperature: the case of pH values. Colloids Surf A Physicochem Eng Asp. 2007; 301: 174–183.
[33] Mohamadnia Z, Zohurian-Mehr MJ, Kabiri K, Jamshidi A, Mohebi H. Ionically cross-linked carrageenan-alginate hydrogel beads. J Biomater Sci Polym Ed. 2008; 19: 47-59.
[34] Durmaz S, Okay O. Acrylamide/2-acrylamido-2-methylpropane sulfonic acid sodium salt-based hydrogels: synthesis and characterization. Polymer. 2000; 41: 3693-3704.
[35] Daneshvara M, Koushaa M, Jokarb N, Koutahzadehb N, Guibal E. Acidic dye biosorption onto marine brown macroalgae: isotherms, kinetic and thermodynamic studies. Chem Eng J. 2012; 204: 225-234.
[36] Singh KP, Mohan D, Sinha S, Tondon GS, Gosh D. Color removal from wastewater using low-cost activated carbon derived from agricultural waste material. Ind Eng Chem Res 2003; 42: 1965-1971.
[37] Mittal A, Mittal J, Malviya A, Gupta VK. Adsorptive removal of hazardous anionic dye “Congo red” from wastewater using waste materials and recovery by desorption. J Colloid Interface Sci. 2009; 340: 16-21.
[38] Gucek A, Sener S, Bilgen S, Mazmanci ML. Adsorption and kinetic studies of cationic and anionic dyes on pyrophyllite from aqueous solutions. J Colloid Interf Sci. 2005; 286: 53-63.
[39] Nandi BK, Goswami A, Das AK, Mondal B,  Purkait MK. Kinetic and equilibrium studies on the adsorption of crystal violet dye using kaolin as an adsorbent. Sep Sci Technol. 2008; 43: 1382-1403.
[40] Kim SH, Lee HS, Ryu DS, Choi SJ, Lee DS. Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli. Korean J Microbiol Biotechnol. 2011; 39: 77-85.