Document Type : Research Paper
Authors
1
Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq
2
Department of Chemistry / Biochemistry Laboratory, College of Education for Pure Science, Ibn Al-Haitham,University of Baghdad, Iraq.
Abstract
The study focused on testing the anti-cancer effects of Ginkgo biloba extract-derived silver nanoparticles (Ag NPs), specifically examining cytokeratin21-1, alpha-1-antitrypsin, and retinol binding protein-1. Synthesis and characterization of Ag NPs from ginkgo biloba extract were conducted, followed by testing different concentrations of bilobetein to evaluate their anti-cancer effects and identify the optimal dose for lowering the concentrations of cytokeratin21-1, human alpha-1-antitrypsin, and retinol binding protein-1.XRD analysis revealed that the crystallite size and crystal structure of Ag-NPs were 29 nm and FCC-like, respectively. FE-SEM images showed spherical and cubic shapes with a particle size of 89 nm. UV-visible spectrum analysis indicated an optical energy gap of 3.5 eV for Ag-NPs.In the experiment, Bilobetein, an active compound in Ginkgo Biloba, and solutions containing varying concentrations of Ag-NPs from Ginkgo Biloba were synthesized. The impact of these solutions on the concentrations of CYFRA21-1, α1AT, and CRBP-1 was investigated to determine the optimal concentration for reducing the protein levels.After adding Bilobetein at a concentration of 8 ppm, the mean α1AT concentration values for control, adenocarcinoma, and small cell lung cancer were 45.16 ± 4.51, 47.72 ± 1.01, and 50.13 ± 1.29, respectively. Similarly, after adding Ag NPs/ginkgo biloba at a concentration of 4 ppm, the α1AT concentration values for control, small cell lung cancer, and adenocarcinoma lung cancer were 44.48 ± 4.88, 45.89 ± 2.18, and 45.16 ± 4.51, respectively.Regarding RBP-1 concentration values, after adding Bilobetein at a concentration of 8 ppm, the mean values for control, adenocarcinoma, .
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