Investigating the Impact of Mycosynthesised Iron Nanoparticles Against Candida albicans Cells and Studying Their Antioxidants Activity

Articles in Press

Document Type : Research Paper

Authors

1 Department of Biology/College of Science/Al-Mustansisiya University

2 College of Science, Mustansiriyah University, Baghdad, Iraq

3 Environmental Research Center/ University of Technology, Baghdad- Iraq

Abstract

Mycosynthesizing Iron nanoparticles (FeNPs) are the focus of this investigation using the endophytic fungus fusarium graminearum isolated from Salvia Rosmarinus and testing the nanoparticle's action against candida albicans cells and antioxidant activity. First, the fungus was isolated and purified on Potato Dextrose Agar (PDA) from Salvia Rosmarinus leaves and stems, after that, fungal biomass was produced using a laboratory-prepared media, and the biomass was introduced to iron salt; Ferrous sulphate (FeSO4.7H2O) solution to mycosynthesis FeNPs. FeNPs were characterised using many techniques. A colour shift from yellowish-orange colour to dark brown is the first indicator of the FeNPs mycosynthesis. FeNPs were detected in UV–vis absorption spectra with a 303 nm band. XRD analyses prove the crystallinity phase of FeNPs with four distinctive peaks; at 2θ values of 24.84°, 33.52°, 39.12°, and 42.31°. FeNPs FTIR spectra showed absorption peaks from 3400 to 406.98 cm1. The FE-SEM scans showed 45.89–80.05 nm spherical particles. The particles revealed a diameter of 86.79 nm (the average) with a size range spanning from 22.23 to 107.7 nm, according to AFM pictures and charts. FE-SEM technique is used to detect the impact of FeNPs on Candida albicans cells with a clear impact on its cell wall. FeNPs showed scavenging activity increased with concentration.

Keywords

Journal of Nanostructures

Articles in Press, Accepted Manuscript
Available Online from 15 March 2024

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