Effect of titanium oxide nanoparticles(TiO2) on the gene expression of Cytochrome P450 gene in Calliphora vicina fly larvae (Diptera: Calliphoridae)

Document Type : Research Paper

Authors

1 Department of biology, College of Education for pure sciences, University of Diyala, Diyala, Iraq

2 Department of Environmental Health Department , College of Environmental Sciences ; Al- Qasim Green Iraq.

Abstract

The effect of titanium dioxide (TiO2) on the gene expression process of the P450 gene was tested in the fly Calliphora vicina (Diptera: Calliphoridae). The results showed that TiO2 was more effective than Delta magic 5% on the gene expression process of P450gene in speciesC. vicina under laboratory conditions.It was found that there was an increase in the mortality rate significantly to 0.31a ±6.78, 0.43a ± 5.10, 0.48b ± 12.54 and 0.65b ± 14.18 individuals when treated with TiO2 during (0, 2, 4, 7 days), compared with 0.42a ± 5.99, 0.38a ± 4.13, 0.55ab ± 10.90, and 0.17b ± 14.18, respectively in the control group. It was noticed that there is a difference between the effect of TiO2 and the pesticide by finding the calculated P value in the statistical tables, since the value of the difference between them in the three concentrations used is P = 0.003, P = 0.1 and P = 0.2, respectively.It was also noted that the significant effect of TiO2 on the pupation stage of the treated larvae compared with the effect of the insecticide, the highestfail ratio of pupation was recorded, reaching 66% of the total number of treated specimens.

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