Synthesis, Characterization, and In-Vitro Investigation of Hexamethylenediamine Modified Graphene Oxide for Controlled Drug Release of Mesalazine

Document Type : Research Paper


1 Department of Chemistry, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran

2 Department of Chemistry, Marand Branch, Islamic Azad University, Marand, Iran

3 Department of Chemistry, Miyaneh Branch, Islamic Azad University, Miyaneh, I.R. Iran


In recent years, researchers have increasingly shown interest in investigating the potential biomedical uses of graphene and its derivatives, owing to their exceptional chemical and physical characteristics.In this study, we developed a drug delivery system by conjugating hexamethylenediamine to the surface of graphene oxide (GO) through an amide bond to facilitate effective loading and targeted release of mesalazine (Mes). The synthesized graphene oxide-hexamethylenediamine-mesalazine (Mes-AGO) was characterized using common analytical techniques including Fourier-transform infrared spectroscopy (FT-IR), Raman spectroscopy, energy-dispersive X-ray analysis (EDX), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The drug release kinetics of Mes from the nanocarrier were investigated at both neutral and acidic pH conditions. The drug release from the Mes-AGO system was pH-dependent, as confirmed by UV analysis. The results showed minimal drug release at neutral pH, while a burst release was observed at acidic pH. Further studies will be carried out to demonstrate the efficiency of drug delivery by the nanocarrier under in vivo conditions.