Effect of Green synthesis of the ZnO/Ag Nanocomposites on MCF-7 cancerous line using High Content Screening

Document Type : Research Paper


1,2,3 College of Education for women, University of Anbar, Iraq


This research was conducted to evaluate the cytotoxicity of Ag:ZnO nanocomposites prepared from C. murale leaves extract on MCF-7 cancer cell line . Four concentrations of 200, 100, 50 and 25 μg/ml were prepared, as the Ag:ZnO Nanocomposites showed the best percentage. Inhibition of the growth of MCF-7 cancer cells. This test included evaluating the effect of Ag:ZnO nanocomposites on the morphology of MCF-7 cancer cells, by detecting changes that occur to cells to measure some cellular indicators including cell permeability, cell number, nuclear content of cells, mitochondrial membrane permeability, and the level of cytochrome C release from cells. The results showed a clear decrease in the number of cells, an increase in nuclear density when the cells were treated with the complex at concentrations of 200 and 100 mg/ml, a significant decrease in the strength of the mitochondrial membrane, and an increase in cytochrome C levels at concentrations 200 and 100 μg/ml, and the results showed the ability of the complex to increase the permeability of the cell membrane. For cells at a concentration of 200 mg/ml because of the damage that occurs in the cell membrane, which leads to an increase in the permeability of cell membranes.