Document Type : Research Paper
Authors
1
Belgorod State University, 85, Pobedy St., Belgorod, 308015, RUSSIA, Email: gorelik@bsu.edu.ru
2
Belgorod State University, 85, Pobedy St., Belgorod, 308015, RUSSIA, Email:
3
Belgorod State University, 85, Pobedy St., Belgorod, 308015, RUSSIA
4
Voronezh State Medical University named after N.N. Burdenko, 10, Studencheskaya st., Voronezh, 394036, RUSSIA
Abstract
In this study, we demonstrated using the carbonized nanogels (CNGs) for their application against infectious bronchitis virus (IBV). In all countries of the world there is bronchopulmonary complications increase caused by hospital infection in the postoperative period. We set the task to create an endobronchitis experimental model. The most common bacteria, found in hospital infection (E coli, Pseudomonas Aeruginosa, Enterobacter, Klebsiella) were selected for this purpose. The study revealed that in order to create a rat endobronchitis model, it was necessary to introduce a mixture of the above mentioned bacteria endobronchially in a concentration of 1-2 x 10 * 8 CFU/ml microbial bodies in the amount of 0.5-0.9 ml. Creation of endobronchitis model in animal experiment (rats) is possible by introduction of endobronchial mixed cultures (E coli, Pseudomonas, Ps. Aeruginosa, Enterobacter, Klebsiella) 1-2 x 10 * 8 CFU/ml microbial bodies in amount of 0.5-0.9 ml. A lower concentration does not cause visually significant inflammatory changes in the presence of untested histological changes in lung and bronchial tissue, and an increase in dosages leads to animal’s death.
Keywords
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