Synthesis, characterization and investigation of phytochemical and anti-inflammatory activities of silver nanoparticles loaded Clerodendrum petasites

Document Type : Research Paper


1 Applied Thai Traditional Medicine, Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand

2 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand


In this study, we synthesized silver nanoparticles (Ag NPs) using the Clerodendrum chinense leaf extract as reducing and stabilizing agent. Inflammation is process of immune responses to microbial infections and cell damage. Root extract of the medicinal plant Clerodendrum petasites (CP), has been widely used as a component of antipyretic and anti-inflammatory remedies. In the present study, we investigated whether CP aerial part (leaf and stem) extracts had the same contents with the root extracts as well as their cytotoxicity and inflammatory effects in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 monocyte/macrophage-like cell model. Analysis of the methanol-extracted CP contents with the high-performance liquid chromatography ultra-violet spectroscopy (HPLC-UV) showed that major phenolic compounds found in the root extract were verbascoside and quercetin, while the aerial parts also additionally contained rosmarinic acid and hispidulin. These CP extracts were not toxic to the cells and able to inhibit production of pro-inflammatory markers, nitric oxide (NO) and prostaglandin E2 (PGE2). In addition, the aerial CP extracts also inhibited the pro-inflammatory cytokines, interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α), implying additional role of rosmarinic acid and hispidulin. In conclusion, the aerial parts of the CP can be used as anti-inflammatory medicinal plants.