Synthesis of a nanoporous molecularly imprinted polymers for dibutyl Phthalate extracted from Trichoderma Harzianum

Document Type : Research Paper

Authors

1 Department of Plant Protection, Faculty of Crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Department of agriculture, mohammad reza Hariri, Science fundation, Babol, Iran

3 Nanotechnology Research Institute, Babol Noshirvani University of Technology, Babol, Mazandaran, Iran

4 Department of Chemistry, Mazandaran University , Babolsar, Iran

10.7508/JNS.2016.03.009

Abstract

In this study, molecularly imprinted polymers were synthesized for dibutyl phthalate as a bioactive chemical compound with antifungal activity which produced by Trichoderma Harzianum (JX1738521). The molecularly imprinted polymers were synthesized via precipitation polymerization method from methacrylic acid, dibutyl phthalate and trimetylolpropantrimethacrylate as a functional monomer, template and cross-linker, respectively. After removal of the template by the eluent from the MIPs, the leached nanoparticles of the MIPs had a good binding capacity as equal 830 mg/g. The polymer particles have been evaluated by field emission scan electron microscopy and Brunauer–Emmett–Teller  techniques. The excellent specific surface area in the molecularly imprinted polymers as equal to 690.301 m2/g comparatively to non-imprinted polymers (ca. 89.894 m2/g), confirms that the nanoporous MIPs were synthesized, successfully. The results indicated that the nanoporous MIPs can be used in solid phase extraction. This is a novel method for separation of the bioactive compounds from fungi secondary metabolites in biological control.

Keywords

References

1. Akpuaka A, Ekwenchi MM, Dashak DA, Dildar A. Gas chromatography-mass spectrometry (GC/MS) analysis of phthalate isolates in n-hexane extract of Azadirachta indica A. Juss (Neem) leaves. J Am Sci. 2012; 8(12): 146-155.
2. Hsouna AB, Trigie M, Mansour RB. Chemical composition, cytotoxicity effect and
antimicrobial activity of Ceratonia silisqua essential oil with preservative effects against listeria inoculated in minced beef meat. Int J Food Microbiol. 2011; 148 (1): 66-72.
3. Syeda FA, Rehman HU, Choudahry MI. Gas Chromatography-Mass Spectrometry
(GC-MS) analysis of petroleum ether extract (oil) and bioassays of crude extract of Iris germanica. Int J Genet Mol Biol. 2011; 3(7): 95-100.
4. Rahdary AA, Sobati ZJ. Chemical composition of essential oil, antibacterial activity and brine shrimp lethality of ethanol extracts from Sedum pallidum. Med Plants Res. 2012; 6(16): 3105-3109.
5. Dorababu M, Joshi MC, Bhawani G. Effect of acqueos extract of neem (Azadirachta indica) leaves on offensive and diffensive gastric mucosal factors in rats. Indian J Physiol Pharmacol. 2006; 50(3): 241-249.
6. Udeinya JI, Shu EN, Uakyi I. An antimalarial neem leaf extract has both schizonticidal and gametocytocidal activities. Am J Ther. 2008; 15(2): 108-110.
7. Strobel G, Daisy B, Castillo U. Natural products from endophytic microorganisms. J Nat Prod. 2004; 67(2): 257–268.
8. Schuster A, Schmoll M. Biology and biotechnology of Trichoderma. Appl Microbiol Biotechnol. 2010; 87: 787-799.
9. Wang X, Zhang L, Li M. Synthesis of a novel imprinted polymeric material for simultaneous recognition of methamidophos and acephate. Adv Polym Technol. 2013; 32 published online, DOI: 10.1002/ adv.21357.
10. Mahony JO, Molinelli A, Nolan K. Anatomy of a successful imprint: analysing the recognition mechanisms of a molecularly imprinted polymer for quercetin. Biosens Bioelectron. 2006; 2: 1383-92.
11. Alvarez-Lorenzo C, Concheiro A. Molecularly imprinted polymer for drug delivery. J Chromatogr B. 2004; 804: 231-245.
12. Haupt K, Mosbach K. Plastic antibodies: developments and applications. Trends
Biotechnol. 1998; 16(11): 468-475.
13. Alexander C, Andersson HS, Andersson LI. Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003. J Mol Recogni. 2006; 19(2): 106-180
14. Amiri A, Ramazani A, Jahanshahi M. Synthesis of a nanostructured molecularly imprinted acrylic acid-based network copolymer as a solid sorbent for the quercetin extraction. J Nanostruct. 2014; 277-283.
15. Kang YF, Duan WP, Li Y. Molecularly imprinted polymers of allyl-β-cyclodextrin and methacrylic acid for the solid-phase extraction of phthalate. Carbohydr Polym. 2012; 88(2): 459-464.
16. He J, Lv RH, Zhu J. Selective solid-phase extraction of dibutyl phthalate from soybean milk using molecular imprinted polymers. Anal Chim Acta. 2010; 661: 215.
17. Habibi R, Rahnama K, Taghinasb M. Evaluating the effectiveness of Native Trichoderma Species in Production of Extracellular Enzymes During Interaction with Plant Pathogenic Fungus Fusarium oxysporum. Appl Res Plant Protection. 2015; 4(2): 73-85.
18. Siddiquee S, Cheong BE, Taslima K. Separation and identification of volatile compounds from liquid cultures of Trichoderma harzianum by GC-MS using three different capillary columns. J Chromatogr Sci. 2012; 50: 358–367.
19. Yang ZH, Chen F, Tang Y. Selective adsorption of di(2-ethylhexyl) phthalate by surface imprinted polymers with modified silica gel as functional support. J Chem Soc Pak. 2015; 37(5): 939-949.
20. Pakade V, Lindahl S, Chimuka L. Molecularly imprinted polymers targeting quercetin in high-temperature aqueous solutions. ‎J Chromatogr A. 2012; 1230: 15–23.
21. Jin Y, Xuan YH, Jin YS. Multi-SPE of Caffeine and Catechin compounds from green tea by Caffeine and (+) Catechin MIPs. J Liq Chromatogr Relat Technol. 2011; 34: 1604–1616.
22. Wang L, Zhang J, Zhao R. Adsorption of 2,4-dichlorophenol on Mn-modified activated carbon prepared from Polygonum orientale Linn. Desalination. 2011; 266: 175-181.
Journal of Nanostructures
Volume 6, Issue 3
July 2016
Pages 245-249

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