University of Kashan Journal of Nanostructures 2251-7871 16 4 2026 10 01 Anti-Cancer Performance of Chitosan/Fe3O4 Nanohybrid for Magnetic Drug Delivery System 4664 4678 115628 10.22052/JNS.2026.04.016 EN Ali Salah Department of Chemistry, College of Sciences, University of Diyala, Iraq Zaid H. Mahmoud Department of Chemistry, College of Sciences, University of Diyala, Iraq Journal Article 2026 04 12 This study aimed to prepare and evaluate chitosan hybrid nanoparticles with a magnetic inorganic component (Fe₃O₄) in different contents (C1-C4), as well as drug-loaded samples (D-C1 and D-C2). The goal was to assess the effect of component ratios on biocompatibility, active compound release, environmental response (pH and magnetic field), and thermal, structural, and magnetic properties. To achieve this, a range of instruments was used, including day 1 and day 5 photo-absorption measurements at concentrations of 50–200 µg/mL to assess cellular response, and cumulative drug-release studies over short time intervals (0–60 minutes). The analysis was prolonged (up to 350 hours) at pH 5.6 and 7.4, with or without a magnetic field (50 mT). Material characterization was performed using EDS, XRD, TGA/DTG, swelling measurements, magnetic (M–H) behavior, and thermal heating. The results showed that biocompatibility was best at lower concentrations (50 and 100 µg/mL), while absorption values gradually decreased at 150 and 200 µg/mL over time. Release was continuous and pH-dependent, with the highest rates in acidic environments, and the magnetic field accelerated release. This was consistent with EDS and XRD characterizations, which confirmed the incorporation of Fe3O4 into the chitosan matrix and an increased contribution of the inorganic phase with increasing Fe3O4 content. TGA/DTG analysis showed improved thermal stability as inorganic component content increased. https://jns.kashanu.ac.ir/article_115628_680a10b46444b34d55412c92fe35e032.pdf