University of Kashan Journal of Nanostructures 2251-7871 15 4 2025 10 01 Al2O3-MgO Hybrid Nanoparticles as an Effective Nanocarrier for Lenalidomide as Anti-Cancer Drug for Treatment of Multiple Myeloma, Smoldering Myeloma, and Myelodysplastic Syndromes 2237 2250 115164 10.22052/JNS.2025.04.063 EN Dilnora Raxmatova Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan Khabibjon Mirzamurodov Bukhara State Medical Institute named after Abu Ali ibn Sino, Bukhara, Uzbekistan Nasiba Kudratova Tashkent State Medical University, Tashkent, Uzbekistan Shodiyor Boboyev Samarkand State University named after Sharof Rashidov, Uzbekistan Xamidullo Fozilov Samarkand State University named after Sharof Rashidov, Uzbekistan Safiya Fayziyeva Bukhara State Pedagogical Institute, Bukhara, Uzbekistan Olim Turakulov Jizzakh branch of the National University of Uzbekistan, Jizzakh, Uzbekistan Makhfuza Ruzmetova Mamun University, Khorezm, Uzbekistan Najmiddin Bakaev Asia International University, Bukhara, Uzbekistan Adolat Jumaboyeva Urgench State Pedagogical Institute, Urgench, Uzbekistan Muxtor Turaev Bukhara State University, Bukhara , Uzbekistan Abdukhakim Abdullaev National Pedagogical University of Uzbekistan, Tashkent, Uzbekistan Anvar Ziyadullayevich Avlokulov Tashkent State University of Economics, Tashkent, Uzbekistan Otabek Mirzayev Urgench State University, Urgench, Uzbekistan Journal Article 2025 05 04 In this study, Al2O3–MgO hybrids were synthesized via aqueous, surfactant-free processing, yielding ≈84% isolated solid and a surface zeta potential of +32 ± 2 mV. LEN was loaded by a 24 h incubation (1:5 drug: carrier w/w) in pH 6.5 buffer, achieving loading capacity (LC) 12.4 ± 0.3% and encapsulation efficiency (EE) 93.1 ± 1.1%. Loading and purity were quantified by HPLC (302 nm; tR 6.8 min) with daily calibration (0.5–50 μg mL−1, R2 ≥ 0.9999). In vitro release used a dialysis-bag method under sink conditions at pH 7.4 and 5.0 (37 °C); cumulative release was UV-Vis monitored (302 nm) and corrected for dilution, revealing near-zero-order kinetics at pH 7.4 and accelerated release at pH 5.0. LEN-loaded Al2O3–MgO demonstrated a 4–6-fold potency advantage over free LEN across MM and MDS cell lines, with negligible toxicity from empty carriers. Confocal imaging showed lysosomal trafficking as the predominant uptake route, aligning with the observed pH-responsive release profile. The system maintains carrier integrity after release and demonstrates high drug-loading efficiency with sustained release, supporting improved therapeutic indices.  https://jns.kashanu.ac.ir/article_115164_baeada39638b4a20837972f637d85988.pdf