University of Kashan Journal of Nanostructures 2251-7871 15 4 2025 10 01 Antibacterial and Anti-Biofilm Efficacy of Novel Nisin-Loaded Silver Nanoparticles Biosynthesized by Fusarium Solani Against Clinical Pathogens 1686 1701 115016 10.22052/JNS.2025.04.012 EN Asia Salih Medical Laboratory Science Department, College of Science, , Charmo University, Sulaymaniyah, Chamchamal, Iraq Mohammed Lateef Hamk Medical Laboratory Science Department, College of Science, , Charmo University, Sulaymaniyah, Chamchamal, Iraq Journal Article 2025 06 11 Currently, nanoparticles, particularly silver nanoparticles, are interesting because of their unique physicochemical attributes and extensive range of biological, catalytic, electrical, and environmental applications. Among various fabrication methods, green synthesis presents an eco-friendlier and more sustainable alternative compared to traditional chemical or physical techniques. This study explores the green synthesis of silver nanoparticles employing the cell-free supernatant derived from Fusarium solani, and the bioconjugation of silver nanoparticles with an antimicrobial peptide nisin was evaluated in a one-pot reaction. The synthesis conditions have been optimized for high yield and favorable nanoparticle characteristics. The optimization results were 9.0 and 72 h for in 1:2 v/v (cell-free supernatant: salt), 1.5 mM of salt. The synthesized nanoparticles were characterized using ultraviolet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy, x-ray diffraction, Fourier transform infrared spectroscopy, energy dispersive x-ray spectroscopy, atomic force microscopy, and zeta potential studies to verify the formation of nanoparticles. Characterization averaged particle sizes of 10.7 nm for silver nanoparticles and 12.8 nm for nisin-loaded silver nanoparticles were found to be quasi-spherical. Both nanoparticles exhibited potent antimicrobial behavior, indicating their potential against pathogenic bacteria. This work emphasizes the efficacy of green-synthesized silver nanoparticles and their nisin-conjugated analogues as effective, low-cost, and environmentally friendly biomedical agents, especially in antimicrobial treatment as well as eliminating biofilms. https://jns.kashanu.ac.ir/article_115016_a7df37a8d556e4e0cadab843185bf8f0.pdf