University of Kashan Journal of Nanostructures 2251-7871 15 4 2025 10 01 Selenium Nanoparticles Mycosynthesis Using the Endophytic Fungi Fusarium graminearum and Investigating its Antioxidants and Cytotoxicity Effect 1606 1617 115010 10.22052/JNS.2025.04.005 EN Amer H. Abbas Department of Applied Science, University of Technology, Baghdad, Iraq College of Science, Mustansiriyah University, Baghdad, Iraq Shatha Ali Shafiq College of Science, Mustansiriyah University, Baghdad, Iraq Azhar M. Haleem Environmental Research Center, University of Technology, Baghdad, Iraq Journal Article 2025 06 28 Mycosynthesizing Selenium nanoparticles (SeNPs) are the focus of this investigation using the endophytic fungus fusarium graminearum isolated from Salvia Rosmarinus and testing the nanoparticle’s cytotoxicity and antioxidant activity. First, the fungus was isolated and purified on Potato Dextrose Agar (PDA) from Salvia Rosmarinus leaves. After that, fungal biomass was produced using a laboratory-prepared media, and the biomass was introduced to sodium selenite solution to mycosynthesised   SeNPs. SeNPs were characterised using many techniques. A colour shift from pale light to deep orange is the first indicator of the SeNPs mycosynthesis. SeNPs were detected in UV–vis absorption spectra with a 273 nm band. XRD analyses prove the crystallinity phase of SeNPS with three distinctive peaks. SeNPs’ FTIR spectra showed absorption peaks from 3400 to 406.98 cm1. The SEM scans showed 53–62 nm spherical particles. The particles revealed a diameter of 83.23 nm (the average) with a size range spanning from 40.22 to 74.87 nm, according to AFM pictures and charts. The synthesised SeNPs were tested for antioxidant and cytotoxic properties. Selenium nanoparticles were tested for their antioxidant capabilities by scavenging 1,1-diphenyl-2-picryl-hydrazyl. Compared to selenium salt, selenium nanoparticles’ scavenging activity increased with concentration. There was a dosage relationship between SeNPs and anticancer properties. https://jns.kashanu.ac.ir/article_115010_d6ac37c4f1ea69518cdde0a2585a3678.pdf