University of Kashan Journal of Nanostructures 2251-7871 15 2 2025 04 01 Multi-Amine Decorated with Gold Nanoparticles Supported on Multi-Walled Carbon Nanotubes (CNT-CPTMS-PEHA-Au) as Novel Nanocomposites for Investigation of Photothermal Chemotherapy 788 800 114984 10.22052/JNS.2025.02.036 EN Balzhan Azimkhanova Department of Biochemistry, NCJSC Asfendiyarov Kazakh National Medical University, Almaty, Kazakhstan Elaaf Fadhil Hassan Department of Clinical Pharmacy, College of Pharmacy, University of Al-Ameed, Karbala, Iraq Sadi Shirshab Warka University College, Iraq Ali Fawzi Al-Hussainy College of Pharmacy, Ahl Al Bayt University, Kerbala, Iraq Safa Jasim Tuama College of Health and Medical Technologies, National University of Science and Technology, Dhi Qar, Iraq Fatima Radhi Department of Dentistry, Al-Manara College For Medical Sciences, Maysan, Iraq Shahad Ahmed Department of Nursing, Al-Zahrawi University College, Karbala, Iraq Fathi Jihad Hammady Mazaya University College, Iraq Akhmedov Farkhod Department of “Nuclear Medicine and Medical Radiology”, Bukhara State Medical Institute, Bukhara, Uzbekistan Norova Khurshida Tashkent State Technical University named after Islam Karimov, Uzbekistan Sharapov Ilhamberdi Ferghana Medical Institute of Public Health, Republic of Uzbekistan SHavkatov Khasan Department of Obstetrics-Gynecology №2, Samarkand State Medical University, Uzbekistan Yoqubov Diyorbek Department of Fruits and Vegetables At the Urganch State University, Uzbekistan Journal Article 2025 01 09 In this study, a novel nanocomposite, CNT-CPTMS-PEHA-Au, was synthesized through a multi-step surface functionalization strategy aimed at enhancing photothermal chemotherapy efficacy. Multi-walled carbon nanotubes (MWCNTs) were first oxidized to introduce functional groups, followed by silanization with 3-chloropropyltrimethoxysilane (3-CPTMS) and subsequent attachment of pentaethylenehexamine (PEHA) to graft amino functionalities. Gold nanoparticles (AuNPs) were then uniformly deposited onto the functionalized CNT surface via in situ reduction of HAuCl₄ with sodium borohydride, resulting in a stable nanocomposite exhibiting well-dispersed AuNPs approximately 40–50 nm in size. Characterization through FE-SEM and FT-IR confirmed the morphological integrity, successful surface modifications, and gold loading. The nanocomposite demonstrated rapid and stable in vitro photothermal conversion, reaching a maximum temperature of 47.8 °C within 10 minutes under 808 nm laser irradiation at 1.5 W/cm², with excellent thermal stability over consecutive cycles. Biological evaluation using MCF-7 cells revealed significant temperature-dependent cytotoxicity, with up to 67% cell death at 50 μg/mL post-irradiation. These results underscore the potential of CNT-CPTMS-PEHA-Au as an efficient, stable, and targeted platform for minimally invasive photothermal cancer therapy, while highlighting challenges related to in vivo translation and scalable synthesis. Future studies should focus on targeting specificity and biocompatibility enhancements for clinical viability. https://jns.kashanu.ac.ir/article_114984_ae70650046a3c014622b40a7730053e9.pdf