University of Kashan Journal of Nanostructures 2251-7871 15 2 2025 04 01 Synergistic Effects of Carbon Nanotubes with Methotrexate for Osteosarcoma: A Review 712 719 114971 10.22052/JNS.2025.02.029 EN Sattarova Aigul Myrzahanovna Department of Chemistry and Biology, Peoples’ Friendship University named after Academician A. Kuatbekov, Shymkent, Republic of Kazakhstan Noor Zuhier Habeeb Department of physics, College of Education for Pure Sciences, University Of Anbar, Iraq Hayder Hamid Abbas Al-Anbari College of Pharmacy, Ahl Al Bayt University, Kerbala, Iraq Bahira Abdul Razzaq Mohammed Al-Hadi University College, Baghdad, Iraq Fatima Radhi Department of Dentistry, Al-Manara College For Medical Sciences, Maysan, Iraq Sami Najaf Bokhoor College of Health and Medical Technologies, National University of Science and Technology, Dhi Qar, Iraq Hatif Abdulrazaq Yasin Department of Medical Laboratories Technology, Al-Nisour University College, Baghdad, Iraq Reem Mohsin Khalaf Mazaya University College, Iraq Karzhaubay Yerassyl Nurlanovich O. Zhanibekov South Kazakhstan Pedagogical University, Shymken, Republic of Kazakhstan Journal Article 2025 01 27 Osteosarcoma, a highly aggressive primary bone cancer, poses significant therapeutic challenges, particularly due to multidrug resistance and limited efficacy of conventional chemotherapy. Methotrexate (MTX), a widely used antineoplastic agent, suffers from poor bioavailability and systemic toxicity, necessitating the development of advanced drug delivery approaches. In this study, carbon nanotubes (CNTs) are explored as nanocarriers to enhance MTX delivery, aiming to improve therapeutic outcomes. CNTs offer unique physicochemical properties, including high surface area, functionalization potential, and efficient cellular uptake, which can facilitate targeted drug transport and sustained release. The synergistic interaction between CNTs and MTX enhances drug bioavailability, minimizes off-target effects, and improves cytotoxic efficacy against osteosarcoma cells. Experimental findings suggest that CNT-based MTX delivery can overcome resistance mechanisms and significantly improve therapeutic precision. This paper reviewed the current advancements in CNT-mediated drug delivery systems for osteosarcoma, summarizing key experimental findings their implications in overcoming therapeutic challenges.  https://jns.kashanu.ac.ir/article_114971_701e83f4e3da80672e6af53a6c75b8aa.pdf