University of Kashan Journal of Nanostructures 2251-7871 15 3 2025 07 01 Synthesis, Characterization of Silver Nanoparticles Using Rosemary Extract and Their Application as Antioxidant, Antifungal and Antibacterial Agents 885 895 114877 10.22052/JNS.2025.03.007 EN Sarah Abdul Raheem Ali Department of physics, College of Education for Pure Sciences, University of Babylon, Babylon, Iraq Rana A. K. Al-Refaia Department of Chemistry, College of Sciences, University of Babylon, Babylon, Iraq Eman Alrikabi Department of Chemistry, College of Sciences, University of Babylon, Babylon, Iraq Khilowd Omran Ali Al-Mussaib Technical College, Al-Furat Al-Awsat Technical University, Iraq Ali Nasir Hussein Department of Biology, College of Sciences, University of Babylon, Babylon, Iraq Ahmed Ali Alkarimi Department of Chemistry, College of Sciences, University of Babylon, Babylon, Iraq Ali Rahman Al-Shujairi Department of Biology, College of Sciences, University of Babylon, Babylon, Iraq Journal Article 2025 03 14 Medical applications frequently use nanoparticles nowadays, due to their nanometer size and having a significant surface area to volume ratio, allowing them to absorb large amounts of drugs and move quickly through the blood. In this study, silver nanoparticles were successfully synthesized using rosemary leaf extract. The nanoparticles were fully characterized using UV-Vis and Fourier transform infrared spectroscopy, confirming their stability and identifying their functional groups. The average size of the prepared Ag-NPs was 32.7 nm, and the crystallinity was evaluated using XRD, SEM, and EDX. Based on the characterization results, the UV-Vis absorption spectrum of Ag-NPs was determined at 400 nm. Moreover, the antioxidant method confirmed that silver-nanoparticles have more significant antioxidant activity as compared to vitamin C . The biosynthesized Ag-NPs displayed notable antifungal properties against M. cannonballus at varying concentrations, resulting in remarkable inhibitory effects of 74%, 74%, and 98% respectively. Furthermore, the antibacterial activity of the prepared silver nanoparticles and plant extracts against S. aureus and E.coli at two different concentrations 2 mg.mL-1 and 4 mg.ml-1 was investigated using the well diffusion method. The zone of inhibition was utilized as an indicator of antibacterial activity. The findings revealed a potent antibacterial impact of the nano-silver particles on the targeted bacterial strains.The presence of bioactive molecules on the surface of silver nanoparticles indicates that Ag-NPs possess robust antioxidant, antifungal, and antibacterial properties. As a result, Ag-NPs show great potential as biocompatible candidates for various pharmacological and therapeutic uses. https://jns.kashanu.ac.ir/article_114877_ab73653560fbd34ac4a8fc09790dd29c.pdf