University of Kashan Journal of Nanostructures 2251-7871 15 2 2025 04 01 Chemical Synthesis of Zinc Oxide Nanoparticles and Their Antimicrobial Potential Against Opportunistic Hospital Pathogens 576 586 114852 10.22052/JNS.2025.02.018 EN Osamah Raji Alwahhash Al-Qadisiyah director education, Ministry of education, Iraq Mehdi El Arbi Higher Institute of Biotechnology, University of Sfax, Tunisia Journal Article 2025 12 13 Bacterial resistance to antibiotics is a growing global challenge. This study investigates the antimicrobial effects of zinc oxide nanoparticles (ZnO-NPs) on clinical strains of Acinetobacter baumannii and Staphylococcus aureus, focusing on their synergy with the antibiotic ciprofloxacin. ZnO-NPs were synthesized and characterized using XRD, FT-IR, and SEM techniques. Their antimicrobial activity was evaluated against both bacteria, and the minimum inhibitory concentration (MIC) was determined. The results demonstrated that ZnO-NPs effectively inhibited bacterial growth, with enhanced effects when combined with ciprofloxacin, particularly against A. baumannii. These findings suggest that ZnO-NPs could reduce antibiotic resistance and offer a promising approach to combating multidrug-resistant bacteria. https://jns.kashanu.ac.ir/article_114852_3e78b45b18c604b061d5aa4de3dc04ee.pdf