University of Kashan Journal of Nanostructures 2251-7871 15 1 2025 01 01 Synthesis, Characterization, and Biological Evaluation of Nano Schiff Base Metal Complexes: Antibacterial and Anticancer Potential Against Breast Cancer (MCF-7) Cells 88 107 114726 10.22052/JNS.2025.01.009 EN Haider Obaid Jamel Department of Chemistry, College of Education, University of Al-Qadisiyah, Iraq Mohammed Shakir Mwaea Ministry of Education, Directorate of Education Wasit, Wasit, Iraq Duha Eidan Department of Chemistry, College of Education, University of Al-Qadisiyah, Iraq Makarim A. Mahdi Department of Chemistry, College of Education, University of Al-Qadisiyah, Iraq Layth Sameer Jasim Department of Chemistry, College of Education, University of Al-Qadisiyah, Iraq Journal Article 2024 09 10 The synthesized nano Schiff base ligand, 2-(((1E,2E)-2-((4-((E)-1-((4,5-dimethylthiazol-2-yl)imino)ethyl)phenyl)imino)-1,2-diphenylethylidene)amino)phenol (DMTDP), and its metal complexes were evaluated for their biological activity and structural properties. DMTDP was synthesized in two steps, producing a compound with 79% yield and molecular formula C33H28N4OS. The metal complexes were formed by reacting DMTDP with various metals, such as Pd(II), Cu(II), Ni(II), Zn(II), Ag(I), and Cd(II), yielding colored complexes. The structural analysis was performed using UV-Vis, FTIR, and XRD spectroscopy, revealing octahedral and square-planar geometries for most metal complexes.  The biological activity of the synthesized compounds was assessed against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The palladium complex exhibited the highest antibacterial activity, while other complexes displayed moderate effectiveness. Cytotoxicity assays using the MCF-7 breast cancer cell line demonstrated that the palladium complex had significant cytotoxic effects, with an IC50 of 23.4 µg/mL. The complex also displayed selectivity, showing a higher IC50 (67.5 µg/mL) in normal cells, indicating its potential as an anticancer agent.  These findings highlight the importance of Schiff base complexes in biomedical applications, suggesting that palladium-based complexes could serve as promising candidates for anticancer treatments. https://jns.kashanu.ac.ir/article_114726_9f7cd92519c9429f539d5e83b716e2f7.pdf