University of Kashan Journal of Nanostructures 2251-7871 14 4 2024 10 01 Development of Sulfadiazine by Nano Method and Study Its Effect Against Multi-Drug Resistant Bacteria 1023 1028 114561 10.22052/JNS.2024.04.003 EN Ali Jaffar Saleem Department of Biology, College of Education for Pure Science, University of Diyala, Iraq Alaa Salim Hamzah Department of Anesthesia Technique, Institute of Medical Technology, Baghdad, Middle Technical University, Baghdad, Ira Mustafa Hammadi Department of Chemistry, College of Education for Pure Science, University of Diyala, Iraq Journal Article 2024 07 03 Nanotechnology-based antibiotic synthesis is one of the most crucial contemporary strategies for preventing antibiotic resistance. Synthesis of nano sulfadiazine antibiotic was nanoscale made by using standard sulfadiazine in this study, Physically, without using any chemicals. The resulting nanocomposite was examined using XRD, EDX, and SEM methods, and their characteristics were contrasted with those of nano sulfadiazine, whose average crystal size was 48.32 nm. The ability of nano sulfadiazine to prevent bacteria growth was examined by measuring the minimum inhibitory concentration of two species of bacteria using an ELISA technique; it was compared to regular sulfadiazine particles. The results of the broth microdilution method with standard sulfadiazine gradient (concentration) ranges of 8-1024μg/ml show the MIC ranging 64-128μg/ml among five MDR P. aeruginosa isolates and five MDR S. aureus isolates. While the results of Nano-sulfadiazine MIC ranged from 16-32μg/ml for P. aeruginosa, isolates and 32μg/ml for S. aureus isolates. https://jns.kashanu.ac.ir/article_114561_4f934e631fcbbe414e02e64a5ab9cb57.pdf