University of Kashan Journal of Nanostructures 2251-7871 14 1 2024 01 01 Magnetic Nanoparticles for Targeted Drug Delivery in a Mouse Model of Breast Cancer 101 108 114509 10.22052/JNS.2024.01.010 EN Faisal Faisal Departement of Biology, Universitas Islam Malang, Indonesia Galiya Tatarinova Docent Department of Anatomy with Physiology Courses, Kazakh-Russian Medical University, Almaty, Kazakhstan Tursunov Doniyor Mukhamadjon Ugli Department of Oncology of the Andijan State Medical Institute, Andijan, Uzbekistan Djurayeva Lola Abdugabbarovna Termez State University, Termez, Uzbekistan Kamalova Dilnavoz Ikhtiyorovna Department of Physics of the Navoi state pedagogical institute, Navoi, Uzbekistan Kadirova Dilbar Normuminovna Termez State University, Termez, Uzbekistan Mustafoqulov Ruzikul Boynazarovich Termez State University, Termez, Uzbekistan I.B. Sapaev Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research University, Tashkent, Uzbekistan University of Tashkent for Applied Sciences, Tashkent, Uzbekistan Western Caspian University, Scientific researcher, Baku, Azerbaijan Rakhmatova Markhabo Rasulovna Department Clinic Pharmacology, Bukhara State Medical University, Uzbekistan Khayitov Jamshid Komil Ugli Department of Fruits and Vegetables, Urganch State University, Uzbekistan Gulrukhsor Jumaeva Aliyorovna Department of Rehabilitation, Folk Medicine and Physical Education, Tashkent Medical Academy, Tashkent, Uzbekistan Mamatkulova Dilrabo Xamidovna Department of Pediatrics and Medical Genetics, Pediatric Faculty, Samarkand State Medical University, Uzbekistan Isarov Oman Risaliyevich Tashkent Institute of Irrigation and Agricultural Mechanization Engineers National Research, University of Uzbekistan, Uzbekistan Journal Article 2023 09 16 Targeted drug delivery using magnetic nanoparticles (MNPs) has emerged as a promising approach for cancer therapy. This study investigates the efficacy of MNPs for targeted delivery of doxorubicin (DOX) in a mouse model of breast cancer. MNPs were synthesized by co-precipitation and functionalized with DOX. The MNP-DOX conjugates were characterized using TEM, XRD, and FTIR. Female BALB/c mice (n=24) were inoculated with 4T1 breast cancer cells. The mice were divided into four groups: control, DOX, MNP, and MNP-DOX. Every 3 days over a 2-week period, intravenous administration of treatments occurred. Monitoring of tumor volume and body weight took place. Histological analysis and immunohistochemical staining for Ki-67 and caspase-3 were performed on tumor tissues. MNP-DOX conjugates were successfully synthesized and characterized, showing effective drug loading and release. Tumor growth was significantly inhibited in the MNP-DOX group compared to the control, DOX, and MNP groups. The MNP-DOX group exhibited the smallest tumor volume (135±28 mm³) at the end of the treatment period. Histological analysis revealed increased necrosis and apoptosis in the MNP-DOX group. Immunohistochemical staining showed reduced Ki-67 expression and increased caspase-3 expression in the MNP-DOX group. MNPs functionalized with DOX demonstrated effective targeted drug delivery and enhanced therapeutic efficacy in a mouse model of breast cancer. The MNP-DOX conjugates significantly inhibited tumor growth, increased necrosis and apoptosis, and modulated Ki-67 and caspase-3 expression. These findings support the potential of MNPs as a promising platform for targeted cancer therapy. Further studies are needed to optimize the MNP formulation and assess its safety and efficacy in clinical settings. https://jns.kashanu.ac.ir/article_114509_3962b9dea7bf19be780864484295f564.pdf