University of Kashan Journal of Nanostructures 2251-7871 15 4 2025 10 01 Eco-Friendly Synthesis of Ginkgo Biloba- Silver Nanoparticles and Bilobetein: Anti-Cancer Effects on serum for iraqi patients of Lung Cancer 2329 2341 114302 10.22052/JNS.2025.04.071 EN Hiba J. Hussein Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq Nijoud Faisal Yousif AL-Saarag Department of Chemistry, Biochemistry Laboratory, College of Education for Pure Science, Ibn Al-Haitham,University of Baghdad, Iraq Ghasak Hashim Sakban Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq Ghufran Kadhim Talal Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq Journal Article 2024 06 08 The study focused on testing the anti-cancer effects of Ginkgo biloba extract-derived silver nanoparticles (Ag NPs), specifically examining cytokeratin21-1, alpha-1-antitrypsin, and retinol binding protein-1. Synthesis and characterization of Ag NPs from ginkgo biloba extract were conducted, followed by testing different concentrations of bilobetein to evaluate their anti-cancer effects and identify the optimal dose for lowering the concentrations of cytokeratin21-1, human alpha-1-antitrypsin, and retinol binding protein-1.XRD analysis revealed that the crystallite size and crystal structure of Ag-NPs were 29 nm and FCC-like, respectively. FE-SEM images showed spherical and cubic shapes with a particle size of 89 nm. UV-visible spectrum analysis indicated an optical energy gap of 3.5 eV for Ag-NPs.In the experiment, Bilobetein, an active compound in Ginkgo Biloba, and solutions containing varying concentrations of Ag-NPs from Ginkgo Biloba were synthesized. The impact of these solutions on the concentrations of CYFRA21-1, α1AT, and CRBP-1 was investigated to determine the optimal concentration for reducing the protein levels.After adding Bilobetein at a concentration of 8 ppm, the mean α1AT concentration values for control, adenocarcinoma, and small cell lung cancer were 45.16 ± 4.51, 47.72 ± 1.01, and 50.13 ± 1.29, respectively. Similarly, after adding Ag NPs/ginkgo biloba at a concentration of 4 ppm, the α1AT concentration values for control, small cell lung cancer, and adenocarcinoma lung cancer were 44.48 ± 4.88, 45.89 ± 2.18, and 45.16 ± 4.51, respectively.Regarding RBP-1 concentration values, after adding Bilobetein at a concentration of 8 ppm, the mean values for control, adenocarcinoma, and small cell lung cancer were 2.22 ± 0.19, 2.28 ± 0.25, and 2.91 ± 0.46, respectively. Conversely, after adding Ag NPs/ginkgo biloba at a concentration of 4 ppm, the RBP-1 concentration values for control, small cell lung cancer, and adenocarcinoma lung cancer were 2.07 ± 0.13, 2.09 ± 0.049, and 2.62 ± 0.149, respectively. https://jns.kashanu.ac.ir/article_114302_e7537dec91bea34c6a1199e2e759ec0a.pdf