University of Kashan Journal of Nanostructures 2251-7871 13 2 2023 04 01 Silver-Chitosan Nanocomposite Prepared with Aqueous Sodium-Hydroxide and Aqueous Acetic Acid Solutions: Characteristics and Their Cytotoxic Effects 397 407 114022 10.22052/JNS.2023.02.010 EN Laya Ebrahimi Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Saeid Hosseinzadeh Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Maryam Montaseri Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Enayat Berizi Nutrition Research Center, Department of Food Hygiene and Quality Control, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. Mohammad Hashem Yousefi Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Jaafar Jalaei Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Mansour Rahsepar Department of Materials Science and Engineering, School of Engineering, Shiraz University, Iran Journal Article 2022 10 24 In this study, cytotoxic effects of silver-chitosan nanocomposites with aqueous sodium hydroxide solution (SCNC-ASHS) and aqueous acetic acid solution (SCNC-AAAS) were evaluated in vitro. The morphology of the synthesized nanoparticles was characterized by Fourier-Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Their cytotoxicities were then evaluated using MTT in concentrations of 1.56 to 400 µg/ml, and Acridine orange/Ethidium bromide (AO/EB) staining test after 24 h and 48 h. Results showed that the highest cytotoxicity was at 400 µg/ml concentration at which SCNC-ASHS respectively showed 80.57% and 84.37% toxicity on Vero and HT-29 cells after 24 h, and 82.20% and 84.84% after 48 h. While, the cytotoxicities for SCNC-AAAS on Vero and HT-29 cell lines were respectively 80.63% and 87.64% after 24 h, and 83.60% and 87.44% after 48 h. The most toxicity on HT-29 cells belonged to SCNC-AAAS with IC50 of 40.4 µg/ml. In the staining test, SCNC-AAAS revealed 41.84% HT-29 cell viability at 25 µg/ml concentration and 37.51% Vero cell viability at 6.25 µg/ml concentration. Generally, by increasing both SCNCs concentrations, the cell viabilities were decreased, and early and late apoptosis and necrosis were increased in AO/AE test. In conclusion, types of nanoparticles, synthesis methods, and different cell lines play considerable roles in inducing cytotoxicity. According to the higher significant cytotoxicity effects of both SCNCs on colon cancerous cells (HT-29) than normal cells (Vero) (p<0.05), it seems that they have anticancer effects; of those SCNC-AAAS displayed the higher effect with the IC50 of 4.4 µg/ml on HT-29 cells. https://jns.kashanu.ac.ir/article_114022_47c3696594516eacbe65867aaf56a1b8.pdf