University of Kashan Journal of Nanostructures 2251-7871 10 3 2020 07 01 Evaluation of Biological Activity of 5-Fluoro-Isatin Thiosemicarbazone Derivatives 509 517 110794 10.22052/JNS.2020.03.007 EN Mohamed Abdulhamid Ganim Ramadan Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University Mehmet Cengiz Baloglu Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University Yasemin Celik Altunoglu Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Kastamonu University Fatma Kandemirli Department of Biomedical Engineering, Faculty of Engineering and Architecture, Kastamonu University Hakan Burhan en Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100 Kütahya, Turkey Ayşenur Aygün en Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100 Kütahya, Turkey Hakan Sayiner Department of Infectious Diseases, Faculty of Medicine, Adiyaman University, Adiyaman, Turkey Filiz Ozyigit Department of Medical Sciences, Faculty of Medicine, Kutahya University of Health Sciences Fatih Sen Sen Research Group, Department of Biochemistry, Faculty of Arts and Science, Dumlupınar University, Evliya Çelebi Campus, 43100 Kütahya, Turkey Journal Article 2019 11 27 Isatin based materials can exhibit a wide range of biological activities including antimicrobial, antiviral, antifungal, anthelmintic, antitumor, anti-HIV, anti-inflammatory, antidepressant, antioxidant, anticonvulsant, antitubercular, analgesic, and central nervous system depressant activities. In this study, four compounds containing 5-Fluoro-isatin thiosemicarbazone with methoxyphenyl or methoxyphenyl in different positions and zinc complexes were evaluated based on their biological activities. Compound 2 was the strongest compound affecting gram-negative bacteria compared to the other compounds. Also, this compound indicated better antimicrobial activity than positive control antibiotics. Besides, compound 3 was the only compound that inhibited the growth of Salmonella spp. such as Salmonella enteritidis ATCC 13076 and Salmonella typhimurium NRRLE 4463. 5-Fluoro-Isatin thiosemicarbazone and its derivatives also showed DNA protection property from moderate to good protections. Among them, compound 4 displayed the highest DNA binding affinity. These compounds possessed a capacity for utilization as drugs or drug additives based on their effects on bacteria strains and DNA binding affinity. https://jns.kashanu.ac.ir/article_110794_98d169c900657895924ba535d1680a4f.pdf