Synthesis and Characterization of Novel Modified and Functionalized Silica Nano-particles for Protein Delivery Applications

Document Type: Research Paper


Department of Chemistry, Azarbaijan Shahid Madani University, Tabriz, Iran


In this study, the synthesis, characterization and controlled release behavior of new Hollow Silica Nano particles (HSNPs) and Magnetic Silica Nano Particles (MSNPs) were studied. Magnetic Silica Nano particles (MSNPs), as drug delivery vehicles, were synthesized through the coating of Fe3O4 nano-crystals with silica layers. The HSNPs were obtained by removal of Fe3O4 templates with hydrochloric acid and then calcinated at the high temperature. In the first step, MSNPs and HSNPs were modified and the amine and aldehyde functional groups were introduced on the silica surfaces. In the next step, positively charged silica nano particles were synthesized through the quaternization of Aminated Silica Nano Particles (ASNP) with sodium monochloroacetate (SMCA). Following characterization, insulin was loaded into the nano particles as a model system which demonstrated a notable sustained drug release. The study shows that the HSNPs could load a higher amount of drug. The release rates from the HSNPs were faster than those of MSNPs.


1. Abbaszad Rafi A, Mahkam M. Preparation of magnetic pH-sensitive microcapsules with an alginate base as colon specific drug delivery systems through an entirely green route. RSC Adv. 2015;5(6):4628-38.
2. Mahkam M, Abbaszad Rafi A, Mohammadzadeh Gheshlaghi L. Preparation of novel pH-sensitive nanocomposites based on ionic-liquid modified montmorillonite for colon specific drug delivery system. Polym Compos. 2014;37(1):182-7.
3. Abbaszad Rafi A, Fakheri F, Mahkam M. Synthesis and preparation of new pH-sensitive nanocomposite and nanocapsule based on “MCM-41/poly methacrylic acid” as drug carriers. Polym Bull. 2016;73(10):2649-59.
4. Bazmi Zeynabad F, Abbaszad Rafi A, Mahkam M, Taheri H. Preparation of New pH-Sensitive Nanocomposites through in situ Copolymerization of Methacrylic Acid with Ionic Liquid-Modified Laponite Clay. Polym Plast Technol Eng. 2016;55(15):1604-12.
5. Gorsd MN, Pizzio LR, Blanco MN. Synthesis and Characterization of Hollow Silica Spheres. Procedia Mater. Sci. 2015;8:567-76.
6. Yang J, Lee J, Kang J, Lee K, Suh J-S, Yoon H-G, et al. Hollow Silica Nanocontainers as Drug Delivery Vehicles. Langmuir. 2008;24(7):3417-21.
7. Wu Q, Li R, Zhao C, Ren J, Du K, Yin B, et al. In vivo evaluation of an anticancer drug delivery system based on heparinized mesoporous silica nanoparticles. RSC Advances. 2015;5(50):40103-10.
8. Abbaszad Rafi A, Mahkam M, Davaran S, Hamishehkar H. A Smart pH-responsive Nano-Carrier as a Drug Delivery System: A hybrid system comprised of mesoporous nanosilica MCM-41 (as a nano-container) & a pH-sensitive polymer (as smart reversible gatekeepers): Preparation, characterization and in vitro release studies of an anti-cancer drug. Eur J Pharm Sci. 2016;93:64-73.
9. Mortazavi Y, Ghoreishi SM. Synthesis of Mesoporous Silica and Modified as a Drug Delivery System of Ibuprofen. J Nanostruct. 2016;6(1):86-89.
10. Slowing I, Viveroescoto J, Wu C, Lin V. Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers. Adv Drug Del Rev. 2008;60(11):1278-88.
11. Trewyn BG, Giri S, Slowing II, Lin VSY. Mesoporous silica nanoparticle based controlled release, drug delivery, and biosensor systems. Chem Commun. 2007(31):3236.
12. Eshaghi A, Mojab M. Hydrophilicity of Silica Nano-Porous Thin Films: Calcination Temperature Effects. J Nanostruct. 2017;7(2):127-133.
13. Hedayati K, Goodarzi M, Ghanbari D. Hydrothermal Synthesis of Fe3O4 Nanoparticles and Flame Resistance Magnetic Poly styrene Nanocomposite. J Nanostruct. 2017;7(1):32-39.
14. Ahmadi Golsefidi M, Abbasi F, Abrodi M, Abbasi Z, yazarlou F. Synthesis, characterization and photocatalytic activity of Fe2O3-TiO2 nanoparticles and nanocomposites. J Nanostruct. 2016;6(1):64-69.
15. Hedayati K, Azarakhsh S, Ghanbari D. Synthesis and magnetic investigation of cobalt ferrite nanoparticles prepared via a simple chemical precipitation method. J Nanostruct. 2016;6(2):127-131.
16. Shahiri Tabarestani M, Rahnama K, Jahanshahi M, Nasrollanejad S, Fatemi MH. Synthesis of a nanoporous molecularly imprinted polymers for dibutyl Phthalate extracted from Trichoderma Harzianum. J Nanostruct. 2016;6(3):245-249.